RNA Preparation and Treatment

C. elegans RNA prep : Michael Koelle ()
Worm RNA Preparation : MGH ()
LiCl RNA Preparation : MGH ()
RNA Preparation : MGH ()
Northern Blots : Michael Koelle ()

DNA Transformation/Library Preparation NMFS/NOAA

Cosmid packaging protocol : NMFS/NOAA ()
Transformation-Competent E. coli preparation rubidium chloride method : NMFS/NOAA ()

Plasmid DNA

Large Scale Plasmid Preparation : MGH ()
Low copy plasmid purification using Qiagen Midi columns : NMFS/NOAA ()
Mini-Prep Procedure For E. coli Plasmid Purification : Chuck Tauer ()
Plasmid miniprepII : GSC ()
Plasmid min iprepI : GSC ()
Genome Sequencing Center Protocol List : ()

• DNA purification using Diatomaceous Earth, (plasmids, cosmids and p1) : NMFS/NOAA
  Mini-preps : NMFS/NOAA ()
  Midi-preps : NMFS/NOAA ()
  Maxi-preps, and all media, solutions : NMFS/NOAA ()
  Introduction : NMFS/NOAA ()
  

Cosmid DNA

Cosmid fingerprinting : GSC ()
Codes for cosmid vectors : GSC ()

Phage DNA

Single stranded template from pBluescript phagemid: : NMFS/NOAA ()
Lambda DNA Miniprep : David Harry ()
Largescale M13 prep : GSC ()
M13 PEG prepII : GSC ()
M13 PEG prepI : GSC ()
M13 helperphage : GSC ()
M13 ThermoMAX prep : GSC ()
M13 RF prep largescale : GSC ()
Preparation of M13 sublibraries : GSC ()
PCR of M13 inserts : GSC ()

Genomic DNA

• Molecular Cytogenetic Genome Screen
  DAPI chromosome identification : ()
  Image analysis : ()
  Image capture : ()
  DNA detection : ()
  Hybridization : ()
  DNA labeling by nick translation : ()
  DNA preparation by cryostom tissue dissection : ()
  Metaphase chromosome preparation : ()
  Comparative Genomic Hybridization : ()
  
• Southern Blots
  Alkaline Southern Blotting Procedure : Suzanne Gerttula ()
  Worm genomic Southern blots : Michael Koelle ()
  
  Worm Genomic DNA : MGH ()
  DNA purification, bacterial-genomic via Qiagen columns : NMFS/NOAA ()
  DNA purification, chromosomal, triton extraction method : NMFS/NOAA ()
  

YACs

Easy YAC Preparation Method : Davies ()

Fragments

DNA isolation from low melting-temperature agarose : NMFS/NOAA ()

DNA Sequencing

Sequencing gels: wedge gel performance without pouring wedge gels : NMFS/NOAA ()
Sequencing gel solutions : NMFS/NOAA ()
Linear amp seqIV Licor : GSC ()
Linear amp seqIII : GSC ()
Linear amp seqII : GSC ()
Linear amp seqI : GSC ()
Terminator reaction clean-up : GSC ()
Sequencing through stop regions of M13 or pUC subclones, and PCR products : GSC ()
Direct Automated Sequencing off Bacterial Genomic DNA : Bruce Rhoe ()

PCR and Related Methods

Site-directed Mutagenesis using PCR : NMFS/NOAA ()
Cloning PCR-Generated DNA Fragments : NMFS/NOAA ()
Core sample PCR: a method to re-PCR unique bands from products of mixed size : NMFS/NOAA ()
Tc1 Deletion PCR Tips : MGH ()
Long PCR Protocol : Church Lab ()
Degenerate PCR Protocol : M. Koelle ()

Protein Electrophoresis

Tricine-polyacrylamide gels for high resolution of small proteins : NMFS/NOAA ()

Protein & Peptide HPLC Columns

PolyLC Part Numbers and Prices : ()
SDS Removal Guard Columns : ()
PolyGLYCOPLEX Complex Carbohydrates Columns : ()
PolyWAX LP Weak Anion Exchange Columns : ()
ALKYL Aspartamide HIC Columns : ()
PolySULFOETHYL Aspartamide Strong Cation Exchange Columns : ()
Polyaspartic Acid Weak Cation Exchange Columns : ()
HILIC and SEC Columns PolySULFOETHYL and PolyHYDROXYETHYL Columns : ()