Worm Protocols
Equipment
Simple EPG Rig :
Avery Lab
()
Laser Ablation System :
Avery Lab
()
Building a Dissecting Stereo-Microscope :
Ambros Lab
()
Microinjections
Preparing for Injections :
Michael Koelle
()
Microinjecting worms :
Michael Koelle
()
Integrating extrachromasomal arrays :
Michael Koelle
()
Stainings
Antibody Staining :
MGH
()
Staining C. elegans for B-galactosidase :
Michael Koelle
()
Dye Filling to Stain Amphid and Phasmid Neurons :
Michael Koelle
()
Phalloidin Staining :
Michael Koelle
()
In situ hybridization :
Geraldine Seydoux and Andy Fire
()
Dauer Protocols
Making Dauers with Pheromone :
MGH
()
Dauer Pheromone Preparation :
MGH
()
General
Worm Breeding for Super Geniuses: A Guide to Genetic Mapping in C. Elegans :
David Fay et al
(Tuesday, July 31, 2001)
Novel soluble-compound chemotaxis assay :
Stephen Wicks
()
Liquid Culture of Worms :
Michael Koelle
()
Liquid Culture of Worms :
Zhou
()
Freezing Worms :
Michael Koelle
()
Cleaning Worm Stocks :
Michael Koelle
()
Floating Worms on Sucrose :
MGH
()
Worm Codon Preferences :
Ambros
()
Development of techniques for primary culture of ltIgtC. eleganslt/Igt embryonic neurons Part 3 :
Laird Bloom
()
Development of techniques for primary culture of ltIgtC. eleganslt/Igt embryonic neurons Part 2 :
Laird Bloom
()
Development of techniques for primary culture of ltIgtC. eleganslt/Igt embryonic neurons Part 1 :
Laird Bloom
()
Mutants
Mapping Mutants using STS Polymorphisms :
Michael Koelle
()
Tc1-insertion display method :
Henri van Leunen
()
EMS-deletion mutagenesis :
Gert Jansen
()
Tc1-insertional mutatgenesis :
Ronald Plasterk
()
Freezing Worms :
Michael Koelle
()
F2 Mutant Screen - non clonal :
Michael Koelle
()
EMS mutagenesis :
Michael Koelle
()
Integrating extrachromasomal arrays :
Michael Koelle
()
Gene Knockout Protocols :
Leon Avery
()
Drugs
Fixation and Permeabilization :
MGH
()
Buffers :
MGH
()
Levamisole Test :
MGH
()